| A: | During oxidation within a cell, all the energy contained in respiratory substrates is not released free into the cell, or in a single step. |
| B: | The energy released by oxidation in respiration is not or rather cannot be used directly but is used to synthesise ATP. |
| A: | pyruvic acid is completely oxidised to carbon dioxide and ethanol. |
| B: | pyruvic acid is reduced to lactic acid. |
| A: | For aerobic respiration to take place within the mitochondria, the final product of glycolysis, pyruvate is transported from the cytoplasm into the mitochondria. |
| B: | The complete oxidation of pyruvate takes place by the stepwise removal of all the hydrogen atoms, leaving three molecules of CO2. |
| C: | The passing on of the electrons removed as part of the hydrogen atoms to molecular O2 results in the simultaneous synthesis of ATP. |
| A: | Electrons from NADH produced in the mitochondrial matrix during citric acid cycle are oxidised by an NADH dehydrogenase (complex I), and electrons are then transferred to ubiquinone located within the inner membrane. |
| B: | Ubiquinone also receives reducing equivalents via FADH2 (complex II) that is generated during oxidation of succinate in the citric acid cycle. |
| C: | Cytochrome c is a small protein attached to the inner surface of the outer membrane and acts as a mobile carrier for transfer of electrons between complexes III and IV. |
| D: | Complex IV refers to cytochrome c oxidase complex containing cytochromes a and a3, and two magnesium centres. |
| 1. | If fatty acids were to be respired they would first be degraded to acetyl CoA and enter the respiratory pathway. |
| 2. | Glycerol would enter the respiratory pathway after being converted to PGAL |
| 3. | Proteins and amino acids cannot enter the respiratory pathway |
| 4. | Because the respiratory pathway is involved in both anabolism and catabolism, it would hence be better to consider the respiratory pathway as an amphibolic pathway rather than as a catabolic one. |