Q. No.
The restriction enzymes most commonly used in rDNA technology are
1.
Type 1 Restriction enzymes
2.
Type II Restriction enzymes
3.
Type III Restriction enzymes
4.
Type IV Restriction enzymes
Restriction enzymes do not act on the the DNA of the Host cell in which they are produced because
| 1. | Host DNA is packed into chromosomes |
| 2. | Restriction enzymes are ineffective on host DNA |
| 3. | Host DNA does not have the restriction site for the Restriction enzymes. |
| 4. | Restriction site of host DNA is methylated. |
A protein is not expressed properly in a diseased tissue. To find out whether the defect is at the level of translation or post translational modifications which techniques would you use ?
| 1. | Southern blotting and South Western blotting |
| 2. | Northern blotting and western blotting |
| 3. | Western blotting and Eastern blotting |
| 4. | Southern and Northern blotting |
Insertional inactivation of a gene helps in
| 1. | Identification of recombinant clones |
| 2. | Identification of deletion mutants |
| 3. | Identification of recombinant transformants |
| 4. | Elimination of suppression mutants |
Biolistic method makes use of microparticles coated with DNA bombarded at cells to be transformed. These particle are made up of
| 1. | Zinc or tungsten | 2. | Silicon or gold |
| 3. | Tungsten or gold | 4. | Selenium or Platinum |
Insertional inactivation of the lac Z gene forms-
| 1. | Blue recombinant colonies |
| 2. | Colourless recombinant colonies |
| 3. | Fluorescent green colonies |
| 4. | There is no relation between the lac Z gene and colour of the colony. |
A reporter enzyme used in biotechnology is-
| 1. | Green Fluorescent Protein |
| 2. | Luciferase |
| 3. | b Galactosidase |
| 4. | All of the above |
Downstream processing does not include
(1) Quality control
(2) Clinical trials
(3) Product purification
(4) Sampling from bioreactor
Which is not a component of the vector pBR322?
| 1. | Chloramphenicol selectable marker |
| 2. | Replication proteins |
| 3. | Origin of replication |
| 4. | Cloning site for BamHI enzyme |
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